Enzyme kits for polyphosphate research

Enzyme kits for polyphosphate research

Measure polyphosphate concentration and chain length.

Measure polyphosphate concentration and chain length.

Phosfinity Quant quantifies total polyP in any sample. Phosfinity ChainQuant does the same and adds average chain length. Both run on a standard 96-well plate reader, in about an hour, with all enzymes, reagents, and standards included.

Ensure consistent, high-precision results every time. Designed for reliable performance across diverse research and industrial workflows.

Trusted by research groups at

Trusted by research groups at

Complete Polyphosphate Analysis Platform

Complete Polyphosphate Analysis Platform

Analysis of the two key numbers

Analysis of the two key numbers

Polyphosphates (polyP) are linear polymers of orthophosphate residues linked by high-energy bonds. Knowing how much is in your sample is only half the picture.

Polyphosphates (polyP) are linear polymers of orthophosphate residues linked by high-energy bonds. Knowing how much is in your sample is only half the picture.

What Are Polyphosphates?

What Are Polyphosphates?

Polyphosphates (polyP) are linear polymers of orthophosphate residues linked by high-energy phosphoanhydride bonds. Found in bacteria, blood, soil, and plants.

Polyphosphates (polyP) are linear polymers of orthophosphate residues linked by high-energy phosphoanhydride bonds. Found in bacteria, blood, soil, and plants.

Why Measure Concentration?

Why Measure Concentration?

Quantifying total polyP reveals phosphate reserves, metabolic states, and key biological processes accurately and reliably — the most fundamental measurement.

Quantifying total polyP reveals phosphate reserves, metabolic states, and key biological processes accurately and reliably — the most fundamental measurement.

Why Measure Chain Length?

Why Measure Chain Length?

Chain length determines polyP function: short chains serve as phosphate donors, while long chains act as energy stores and chelators. Identical concentration, very different biology.

Chain length determines polyP function: short chains serve as phosphate donors, while long chains act as energy stores and chelators. Identical concentration, very different biology.

Phosfinity Quant

Phosfinity Quant

How much polyP?

How much polyP?

Chain length

Chain length

How long are the chains?

How long are the chains?

Phosfinity ChainQuant

Phosfinity ChainQuant

Both, in one workflow

Both, in one workflow

ChainQuant does everything Quant does, and adds average chain length.

ChainQuant does everything Quant does, and adds average chain length.

You never need both kits to get both numbers; just choose the kit that answers your question.

You never need both kits to get both numbers; just choose the kit that answers your question.

Our Products

Our Products

Two complementary enzyme-based kits delivering complete polyphosphate insight from concentration to chain length

Two complementary enzyme-based kits delivering complete polyphosphate insight from concentration to chain length

PHOSFINITY QUANT

PHOSFINITY QUANT

Polyphosphate Quantification Kit

The gold standard for research-grade polyphosphate quantification. Enzymatic degradation to orthophosphate, detected colorimetrically at 882 nm. No enzyme purification, no daily reagent preparation.

The gold standard for research-grade polyphosphate quantification. Enzymatic degradation to orthophosphate, detected colorimetrically at 882 nm. No enzyme purification, no daily reagent preparation.

WHY LABS SWITCH TO IT

WHY LABS SWITCH TO IT

Reproducible between operators and between days eliminates the variability of self-prepared assays

Reproducible between operators and between days eliminates the variability of self-prepared assays

100 reactions per kit; up to 100 samples measured in a single pass

100 reactions per kit; up to 100 samples measured in a single pass

~1 hour at room temperature, start to result

~1 hour at room temperature, start to result

200 µL sample volume at 100 µM polyP as monomer

200 µL sample volume at 100 µM polyP as monomer

ATP, DNA, RNA and NaCl do not interfere with the measurement

ATP, DNA, RNA and NaCl do not interfere with the measurement

Positive and negative controls included; free Excel analysis sheet

Positive and negative controls included; free Excel analysis sheet



TYPICAL USE

Checking polyP extraction yield from blood, soil, water, plants and microorganisms

Checking polyP extraction yield from blood, soil, water, plants and microorganisms

Screening strains or conditions for polyP accumulation

Screening strains or conditions for polyP accumulation

QC on chemically produced polyP

QC on chemically produced polyP

PHOSFINITY CHAINQUANT

PHOSFINITY CHAINQUANT

Polyphosphate Chain Length Analysis Kit

The world's first research-grade kit for accurate polyphosphate chain length analysis and concentration measurement, both values, one workflow.

The world's first research-grade kit for accurate polyphosphate chain length analysis and concentration measurement, both values, one workflow.

WHY CHAIN LENGTH CHANGES YOUR CONCLUSION

WHY CHAIN LENGTH CHANGES YOUR CONCLUSION

Differentiating chain length is critical: size, not concentration, defines polyphosphate’s specific biological activity.

Differentiating chain length is critical: size, not concentration, defines polyphosphate’s specific biological activity.

Returns chain length and concentration values from one 96-well workflow, no additional steps

Returns chain length and concentration values from one 96-well workflow, no additional steps

Quantification range 5–200 µM polyP as monomer, irrespective of chain length

Quantification range 5–200 µM polyP as monomer, irrespective of chain length

Validated on blood, soil, plant, microbial and water samples

Validated on blood, soil, plant, microbial and water samples

200 µL sample volume; same plate reader, same ~1 hour run

200 µL sample volume; same plate reader, same ~1 hour run

TYPICAL USE

TYPICAL USE

Characterising polyP produced by polyphosphate kinase variants

Characterising polyP produced by polyphosphate kinase variants

Comparing chain length across strains, growth phases or process conditions

Comparing chain length across strains, growth phases or process conditions

Specifying and verifying chain length in industrial polyP production

Specifying and verifying chain length in industrial polyP production

Which Kit Do You Need?

Which Kit Do You Need?

Side-by-side comparison

Side-by-side comparison

Feature
Phosfinity Quant
Phosfinity ChainQuant
What it measures
Measures polyP concentration
Included
Included
Measures average chain length
Included
2-in-1 measurement
ChainQuant only
SPECIFICATIONS
Quantification range
5–200 µM polyP as monomer
5–200 µM polyP as monomer
Sample volume
200 µL
200 µL
Reactions per kit
100
100
Run time
~1 hour
~1 hour
WORKFLOW & COMPATIBILITY
96-well plate format
Included
Included
Requires only a plate reader
Included
Included
Works with any chain length
Included
Included
Tolerates contaminants (ATP, DNA, RNA, NaCl)
Included
Included
Free Excel analysis sheet
Included
Included
BEST FOR
Choose when
Concentration only. Use for screening, extraction yield, routine monitoring
Chain length matters to your biology or process spec

How it works

How it works

1. Sample & Preparation

1. Sample & Preparation

Collect and prepare samples from diverse biological and environmental sources blood, soil, water, plants, microorganisms.

Collect and prepare samples from diverse biological and environmental sources blood, soil, water, plants, microorganisms.

2. Polyphosphate Analysis

2. Polyphosphate Analysis

Measure concentration and chain length using Phosfinity Quant and ChainQuant kits on a standard 96-well plate reader.

Measure concentration and chain length using Phosfinity Quant and ChainQuant kits on a standard 96-well plate reader.

3. Scientific Insights

3. Scientific Insights

Generate reliable, reproducible data to support research conclusions and informed decisions.

Generate reliable, reproducible data to support research conclusions and informed decisions.

Applications

Applications

Where Phosfinity kits are used

Where Phosfinity kits are used

Phosfinity kits are used across a wide range of research and industrial applications involving polyphosphate analysis.

Phosfinity kits are used across a wide range of research and industrial applications involving polyphosphate analysis.

Microbiology

Study polyphosphate metabolism in bacterial systems and microbial communities. Screen strains for polyP accumulation.

Study polyphosphate metabolism in bacterial systems and microbial communities. Screen strains for polyP accumulation.

Enzymology

Analyse enzyme activity related to polyphosphate synthesis and degradation. Characterise kinase variants by chain length output.

Analyse enzyme activity related to polyphosphate synthesis and degradation. Characterise kinase variants by chain length output.

Industrial Biotechnology

Monitor polyphosphate processes in fermentation and bioproduction systems. Specify and verify chain length in manufactured polyP.

Monitor polyphosphate processes in fermentation and bioproduction systems. Specify and verify chain length in manufactured polyP.

Environmental Research

Investigate polyphosphate roles in soil, water, and ecological systems. Compatible with complex sample matrices.

Investigate polyphosphate roles in soil, water, and ecological systems. Compatible with complex sample matrices.

Academic Research

Support fundamental research across universities and research institutes. Cited in peer-reviewed publications.

Support fundamental research across universities and research institutes. Cited in peer-reviewed publications.

Pharma & Life Sciences

Enable advanced research in cellular processes, metabolic pathways, and blood coagulation involving polyphosphates.

Enable advanced research in cellular processes, metabolic pathways, and blood coagulation involving polyphosphates.

Why Phosfinity

Why Phosfinity

Phosfinity was co developed with Prof. Dr. Lars M. Blank and Dr. Jonas J. Christ of the institute of Applied Microbiology at RWTH Aachen, two of the field's authorities of polyP analytics.

Phosfinity was co developed with Prof. Dr. Lars M. Blank and Dr. Jonas J. Christ of the institute of Applied Microbiology at RWTH Aachen, two of the field's authorities of polyP analytics.

REPRODUCIBILITY

REPRODUCIBILITY

Pre-validated enzyme reagents, positive and negative controls in every kit, and a free Excel analysis sheet so every lab calculates results the same way.

Pre-validated enzyme reagents, positive and negative controls in every kit, and a free Excel analysis sheet so every lab calculates results the same way.

SCIENTIFIC RIGOUR

SCIENTIFIC RIGOUR

Used at research institutions across Europe, North America and Oceania. Cited in peer-reviewed publications. The gold standard for polyP quantification.

Used at research institutions across Europe, North America and Oceania. Cited in peer-reviewed publications. The gold standard for polyP quantification.

SIMPLICITY

SIMPLICITY

Any lab with a standard 96-well plate reader can run Phosfinity. No need to purify your own enzyme reagents or prepare fresh solutions daily.

Any lab with a standard 96-well plate reader can run Phosfinity. No need to purify your own enzyme reagents or prepare fresh solutions daily.

EXPERT SUPPORT

EXPERT SUPPORT

Aminoverse scientists answer protocol questions directly. We help you adapt the assay to your specific sample matrix and research context.

Aminoverse scientists answer protocol questions directly. We help you adapt the assay to your specific sample matrix and research context.

Built on scientific expertise and trusted by leading research institutions

Built on scientific expertise and trusted by leading research institutions

Reliable calibration

020651101552000.00.51.01.52.02.5PolyP concentration (µM)Absorption at 882 nm
2021
2022
2023
2024
2025
2026
Best batch per year by R² · Raw absorbance values (no blank subtraction) · Linear regression trendlines

High-resolution chain length

Validated against the gold standard

Validated against the gold standard

ChainQuant agrees with ³¹P NMR within 4% on average and significantly outperforms PAGE

ChainQuant agrees with ³¹P NMR within 4% on average and significantly outperforms PAGE

ChainQuant results align closely with both ³¹P NMR and end-group titration, while PAGE-based estimates diverge substantially from all three methods.

ChainQuant results align closely with both ³¹P NMR and end-group titration, while PAGE-based estimates diverge substantially from all three methods.

−4%

−4%

Avg. deviation vs. ³¹P NMR

Avg. deviation vs. ³¹P NMR

14%

14%

Avg. deviation vs. end-group titration

Avg. deviation vs. end-group titration

348%

348%

Avg. deviation of PAGE vs. ChainQuant

Avg. deviation of PAGE vs. ChainQuant

Testimonials

Testimonials

University of Stuttgart, Germany

Prof. Dr. Dieter Jendrossek

"The PHOSFINITY Quant kit is a time-saving alternative to self-prepared assays. Easy to use and ensures reproducible results."

"The PHOSFINITY Quant kit is a time-saving alternative to self-prepared assays. Easy to use and ensures reproducible results."

University of Alabama, USA

Prof. Dr. Michael J. Gray

The PHOSFINITY Quant kit is a huge advance for the field it lets labs make accurate and specific polyP measurements quickly without needing to purify their own enzyme reagents

The PHOSFINITY Quant kit is a huge advance for the field it lets labs make accurate and specific polyP measurements quickly without needing to purify their own enzyme reagents

Request a Quote

Request a Quote

Tell us about your research needs and our team will get back to you with the right Phosfinity solution, with pricing, lead time and any technical details you need.

Tell us about your research needs and our team will get back to you with the right Phosfinity solution, with pricing, lead time and any technical details you need.

Response within two business days

Response within two business days

Pricing and lead time included

Pricing and lead time included

Technical support from Aminoverse scientists

Technical support from Aminoverse scientists

Guidance on which kit fits your workflow

Guidance on which kit fits your workflow

*Phosfinity is not sold through an online checkout. Every quote is tailored to your research context. A Phosfinity specialist replies within two business days.

*Phosfinity is not sold through an online checkout. Every quote is tailored to your research context. A Phosfinity specialist replies within two business days.

Powered by Aminoverse

Powered by Aminoverse

Aminoverse develops and manufactures Phosfinity. As a CRO for enzyme projects, Aminoverse specializes in enzyme discovery, enzyme engineering, enzyme assay development, and enzyme production. With more than 6 years experience in the enzyme field the Phosfinity kits represent how Aminoverse turns validated assays into robust and ready-to-use kits.

Ensure consistent, high-precision results every time. Designed for reliable performance across diverse research and industrial workflows.

Every Phosfinity kit is backed by Aminoverse's enzyme production infrastructure and quality standards.

Ensure consistent, high-precision results every time. Designed for reliable performance across diverse research and industrial workflows.

Ready to discuss your research needs?

Get in touch with the team to request a tailored quote for Phosfinity Quant, ChainQuant, or both.

Ready to discuss your research needs?

Get in touch with the team to request a tailored quote for Phosfinity Quant, ChainQuant, or both.

Ready to discuss your research needs?

Get in touch with the team to request a tailored quote for Phosfinity Quant, ChainQuant, or both.